Polyunsaturated fatty acid biosynthesis is involved in phenylephrine-mediated calcium release in vascular smooth muscle cells

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• 作者：Nicola A. Irvine^a ; Karen A. Lillycrop^b ; Barbara Fielding^c ; Christopher Torrens^a ; Mark A. Hanson^a ; Graham C. Burdge^a ; ^{g.c.burdge@soton.ac.uk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Polyunsaturated fatty acids ; Vascular smooth muscle cells ; Calcium
• 刊名：Prostaglandins, Leukotrienes and Essential Fatty Acids
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：101
• 期：Complete
• 页码：31-39
• 全文大小：1328 K

文摘

Stimulation of vascular smooth muscle (VSM) 伪₁-adrenoceptors induces myosin phosphorylation and vasoconstriction via mobilisation of intracellular calcium and production of specific eicosanoids. Polyunsaturated fatty acid (PUFA) biosynthesis in VSM cells is involved, although the precise mechanism is not known. To address this, we characterised PUFA biosynthesis in VSM cells and determined its role in intracellular calcium release and eicosanoid production. Murine VSM cells converted 18:2n-6 to longer chain PUFA including 22:5n-6. 螖6 (D6d) and 螖5 (D5d) desaturase, and elongase (Elovl) 5 were expressed. Elovl2 was not detected in human, mouse or rat VSM cells, or in rat or mouse aortae, but tit was not associated with hypermethylation of its promoter. D6d or D5d inhibition reduced 18:3n-6 and 20:4n-6 synthesis, respectively, and induced concentration-related decrease in phenylephrine-mediated calcium release, and in PGE₂ and PGF_2伪 secretion. Together these findings suggest that PUFA biosynthesis in VSM cells is involved in calcium release associated with vasoconstriction.