Nitrated fatty acids: synthesis and measurement

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• 作者：Steven R. Woodcock ; Gustavo Bonacci ; Stacy L. Gelhaus ; Francisco J. Schopfer ; ^{fjs2@pitt.edu}
• 关键词：Nitrated fatty acid ; Nitration ; Mass spectrometry ; Organic synthesis ; Quantification ; Free radicals
• 刊名：Free Radical Biology and Medicine
• 出版年：2013
• 出版时间：June, 2013
• 年：2013
• 卷：59
• 期：Complete
• 页码：14-26
• 全文大小：652 K

文摘

Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography-mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed.