Cytochrome P450s from Cynara cardunculus L. CYP71AV9 and CYP71BL5, catalyze distinct hydroxylations in the sesquiterpene lactone biosynthetic pathway

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Cytochrome P450s from Cynara cardunculus L. CYP71AV9 and CYP71BL5, catalyze distinct hydroxylations in the sesquiterpene lactone biosynthetic pathway

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作者：Kaouthar Eljounaidi^a ; Katarina Cankar^b ; ^c ; Cinzia Comino^a ; Andrea Moglia^a ; Alain Hehn^d ; ^e ; Fré ; dé ; ric Bourgaud^d ; ^e ; Harro Bouwmeester^c ; Barbara Menin^f ; Sergio Lanteri^a ; Jules Beekwilder^b ; ^{jules.beekwilder@wur.nl" class="auth_mail}
关键词：Cynara cardunculus ; Sesquiterpene lactones ; P450 enzymes ; Germacrene A ; N. benthamiana transient expression ; Costunolide
刊名：Plant Science
出版年：June 2014
年：2014
卷：223
期：Complete
页码：59-68
全文大小：1538 K

文摘

•: Two P450 genes (i.e. CYP71AV9 and CYP71BL) involved in sesquiterpene lactone pathway have been isolated in globe artichoke.
•: CYP71BL5 and CYP71AV9 co-expressed with GAS led to biosynthesis of the free costunolide in yeast and costunolide conjugates in N. benthamiana.
•: CYP71AV9 converts also non-natural substrates, amorpha-4,11-diene, (+)-germacrene D and cascarilladiene, to oxidized products.

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