Hepatic stellate cells retain the capacity to synthesize retinyl esters and to store neutral lipids in small lipid droplets in the absence of LRAT

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• 作者：Mokrish Ajat¹ ; ² ; Martijn Molenaar¹ ; Jos F.H.M. Brouwers ; Arie B. Vaandrager ; Martin Houweling ; J. Bernd Helms ; ^{j.b.helms@uu.nl}
• 关键词：Hepatic stellate cells ; LRAT ; DGAT1 ; Retinyl esters ; Lipid droplets ; Lipidomics
• 刊名：Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1862
• 期：2
• 页码：176-187
• 全文大小：1565 K
• 卷排序：1862

文摘

A modified LC–MS/MS procedure allows quantification of all major retinyl ester species. Quiescent hepatic stellate cells synthesize predominantly retinyl palmitate using LRAT. In the absence of LRAT, quiescent hepatic stellate cells synthesize retinyl esters using DGAT1. Quiescent hepatic stellate cells retain the capacity to store retinyl esters in lipid droplets.