Construction and characterisation of O139 cholera vaccine candidates

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• 作者：Led&oacute ; n ; Talena ; Valle ; Edgar ; Valmaseda ; Tania ; Cedré ; Barbara ; Campos ; Javier ; et. al.
• 关键词：O139 cholera vaccine ; hapA mutants ; thyA mutants
• 刊名：Vaccine
• 出版年：2003
• 出版时间：March 7, 2003
• 年：2003
• 卷：21
• 期：11-12
• 页码：1282-1291
• 全文大小：176 K

文摘

The hemagglutinin/protease (HA/P) seems to be an attractive locus for the insertion of heterologous tags in live cholera vaccine strains. A ΔCTXΦ spontaneous mutant derived from a pathogenic strain of O139 Vibrio cholerae was sequentially manipulated to obtain hapA∷celA derivatives which were later improved in their environmental safety by means of a thyA mutation. All the strains here obtained showed similar phenotypes in traits known to be remarkable for live cholera vaccines irrespective of their motility phenotypes, although the hapA mutants had a 10-fold decrease in their colonisation capacity compared with their parental strains in the infant mouse cholera model. However, the subsequent thyA mutation did not affect their colonisation properties in the same model. These preliminary results pave the way for further clinical assays to confirm the possibilities of these vaccine prototypes as safe and effective tools for the prevention of O139 cholera.