Novel preloaded resins for solid-phase biotinylation of carboxylic acids

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• 作者：Nadezda  ; Cankarova ; Petr  ; Funk ; Jan  ; Hlavac ; Miroslav  ; Soural  ; ;   ; ^{souralm@seznam.cz} ;   ; ^{soural@orgchem.upol.cz}
• 关键词：Solid-phase synthesis ; Biotine ; PEG linker ; Acylation ; Affinity chromatography
• 刊名：Tetrahedron Letters
• 出版年：2011
• 出版时间：2 November 2011
• 年：2011
• 卷：52
• 期：44
• 页码：5782-5788
• 全文大小：834 K

文摘

Use of solid-phase synthesis for the derivatization of carboxylic acids with biotinylated spacers consisting of ethylenoxy units is described. An aminomethylated resin provided with an acid-labile aldehyde linker is used as the polymer support and three different systems with a reactive amino group are introduced. Acylation of each system was tested with a set of model carboxylic acids and afforded crude products of excellent purity. The preloaded resins are similar to the Biotin-PEG-NovaTagTM resin but offer several advantages including simple elongation of the spacer arm. The protocols described represent a very efficient way of modifying compounds to obtain ligands for affinity chromatography studies.