Sequential on-line C-terminal sequencing of peptides based on carboxypeptidase Y digestion and optically gated capillary electrophoresis with laser-induced fluorescence detection

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Sequential on-line C-terminal sequencing of peptides based on carboxypeptidase Y digestion and optically gated capillary electrophoresis with laser-induced fluorescence detection

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作者：Miaomiao Tian¹ ; Ning Zhang¹ ; Xiaoxia Liu ; Liping Guo ; Li Yang ; ^{yangl330@nenu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
关键词：C-terminal sequencing ; Peptide ; Carboxypeptidase Y ; Capillary electrophoresis
刊名：Journal of Chromatography A
出版年：2016
出版时间：12 August 2016
年：2016
卷：1459
期：Complete
页码：152-159
全文大小：2070 K

文摘

•: A novel method for on-line C-terminal sequencing of peptides has been developed.
•: Released AAs are sequentially monitored during the entire progress of CPY digestion.
•: Sequence of a peptide can be determined with just one OGCE run.
•: The method exhibits high repeatability and efficiency for peptide sequencing.

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