Accurate quantitation of choline and ethanolamine plasmalogen molecular species in human plasma by liquid chromatography-tandem mass spectrometry

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• 作者：Yurika Otoki^a ; Shunji Kato^a ; Fumiko Kimura^a ; Katsutoshi Furukawa^b ; Shinji Yamashita^c ; Hiroyuki Arai^b ; Teruo Miyazawa^d ; ^e ; Kiyotaka Nakagawa^a ; ^{nkgw@m.tohoku.ac.jp}
• 关键词：AD ; Alzheimer&rsquo ; s disease ; CE ; collision energy ; CV ; coefficient of variation ; CXP ; collision cell exit potential ; DP ; declustering potential ; EP ; entrance potential ; LC&ndash ; MS/MS ; liquid chromatography&ndash ; tandem mass spectrometry ; LLE ; liquid-liquid extraction ; LOD ; limit of detection ; LOQ ; limit of quantification ; MMSE ; mini mental state examination ; MRM ; multiple reaction monitoring ; PC ; phosphatidylcholine ; PC-Pls ; phosphatidylcholine plasmalogen ; PE ; phosphatidylethanolamine ; PE-Pl
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：5 February 2017
• 年：2017
• 卷：134
• 期：Complete
• 页码：77-85
• 全文大小：947 K
• 卷排序：134

文摘

Novel accurate plasmalogen quantitation method by LC–MS/MS was reported. In the presence of sodium, selective and sensitive detection was achieved. This extraction method from human plasma was simple, accuracy and precise. Enough separation by LC enables to avoiding matrix effects. The method has been successfully applied to quantitate clinical samples.