Development and application of a new Silent reporter system to quantitate the activity of enhancer elements in the type II Collagen Gene

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Development and application of a new Silent reporter system to quantitate the activity of enhancer elements in the type II Collagen Gene

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作者：Kazuo Ito^a ; ^b ; Tamayuki Shinomura^a ; ^{t.shinomura.trg@tmd.ac.jp" class="auth_mail" title="E-mail the corresponding author}
关键词：βgyg ; a β-galactosidase-hygromycin phosphotransferase fusion gene ; RCS ; an established cell line from the Swarm rat chondrosarcoma ; X-gal ; 5-bromo-4-chloro-3-indolyl-β-D-galactoside ; Col2a1 ; a gene encoding type II collagen alpha 1 chain ; PCR ; polymerase chain reaction
刊名：Gene
出版年：2016
出版时间：1 July 2016
年：2016
卷：585
期：1
页码：13-21
全文大小：1385 K

文摘

•: We developed a novel reporter assay system to quantify enhancer activities.
•: 10">The system is based on a silent reporter gene integrated in a chromosome.
•: 15">Activities of different enhancer elements were systematically compared.
•: Col2a1 enhancers E1 and E2 exhibit additive and synergistic effect, respectively.

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