Use of Nicotiana tabacum transplastomic plants engineered to express a His-tagged CP47 for the isolation of functional photosystem II core complexes

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• 作者：Cristina Pagliano^a ; ^{cristina.pagliano@polito.it} ; Luca Bersanini^b ; ¹ ; Rino Cella^b ; Paolo Longoni^b ; ² ; Laura Pantaleoni^b ; ³ ; Abhishek Dass^c ; Sadhu Leelavathi^c ; Vanga Siva Reddy^c ; ^{vsreddy@icgeb.res.in}
• 关键词：Biolistic chloroplast transformation ; CP47 His-tag ; Photosystem II ; Transplastomic tobacco plants
• 刊名：Plant Physiology and Biochemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：111
• 期：Complete
• 页码：266-273
• 全文大小：1041 K
• 卷排序：111

文摘

Generation of tobacco transplastomic plants engineered to express His-tagged CP47. Isolation of a functional PSII core complex from C-term His-tag CP47 mutants. Applicability of a one-step purification method for structural-functional studies.