Preparation of RuBpy-doped Silica Fluorescent Nanoprobes and Their Applications to the Recognition of Liver Cancer Cells

详细信息    查看全文

• 作者：Min-Yan CHEN ; Ze-Zhong CHEN ; Wei WANG ; Lian ZHU ; Hong-Wu TANG ; ^{hwtang@whu.edu.cn" class="auth_mail} ; Dai-Wen PANG
• 关键词：Dye-doped silica nanopartilcles ; Bioconjugation ; Liver cancer cells ; Carcinoembryonic antigen (CEA)
• 刊名：Chinese Journal of Analytical Chemistry
• 出版年：March, 2014
• 年：2014
• 卷：42
• 期：3
• 页码：326-331
• 全文大小：1018 K

文摘

Two different functional groups modified RuBpy-doped silica fluorescent nanoprobes Probe A and B that conjugated with avidin were prepared for the recognition of liver cancer cells. Firstly RuBpy-doped silica nanoparticles were synthesized by reverse microemulsion and then modified with different functional groups. Probe A was prepared by the conjugation of avidin with carboxyl modified nanoparticles through covalent binding using EDC/sulfo-NHS, whereas Probe B was prepared by the conjugation of avidin with the PEG linkers on the surface nanoparticles using cyanogen bromide method. Therefore, different from Probe A, Probe B was obtained by coupling avidin to the nanoparticles through long-chain PEG molecules. The two probes were incubated with liver cancer cells respectively, and microscopic fluorescence imaging showed that Probe B which contained PEG molecules was more effectively applied for the recognition of tumor marker carcinoembryonic antigen (CEA) in liver cancer cells.