Natural lactoylcholine (LaCh) was first isolated from lactic-fermented food.
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Acetylcholine (AcCh) was also isolated from lactic-fermented food.
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The D/L ratio of LaCh and lactic acid was similar (1.6 and 1.7, respectively).
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class="smallcaps">d-LaCh caused vasorelaxation of thoracic aortas of spontaneously hypertensive rats.
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LaCh and AcCh could be new functional ingredients in lactic-fermented foods.
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