Complementarity of SOMAscan to LC-MS/MS and RNA-seq for quantitative profiling of human embryonic and mesenchymal stem cells

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Complementarity of SOMAscan to LC-MS/MS and RNA-seq for quantitative profiling of human embryonic and mesenchymal stem cells

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作者：Anja M. Billing ^{anb2061@qatar-med.cornell.edu" class="auth_mail" title="E-mail the corresponding author} ; Hisham Ben Hamidane ^{hbh2002@qatar-med.cornell.edu" class="auth_mail" title="E-mail the corresponding author} ; Aditya M. Bhagwat ^{adb2018@qatar-med.cornell.edu" class="auth_mail" title="E-mail the corresponding author} ; Richard J. Cotton ^{rjc2003@qatar-med.cornell.edu" class="auth_mail" title="E-mail the corresponding author} ; Shaima S. Dib ^{ssd2002@qatar-med.cornell.edu" class="auth_mail" title="E-mail the corresponding author} ; Pankaj Kumar ^{pankaj123@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Shahina Hayat ^{shh2026@qatar-med.cornell.edu" class="auth_mail" title="E-mail the corresponding author} ; Neha Goswami ^{neg2007@qatar-med.cornell.edu" class="auth_mail" title="E-mail the corresponding author} ; Karsten Suhre ^{kas2049@qatar-med.cornell.edu" class="auth_mail" title="E-mail the corresponding author} ; Arash Rafii ^{jat2021@qatar-med.cornell.edu" class="auth_mail" title="E-mail the corresponding author} ; Johannes Graumann ; ^{jog2030@qatar-med.cornell.edu" class="auth_mail" title="E-mail the corresponding author}
关键词：ESC ; embryonic stem cells ; ESC-MSC ; embryonic stem cell-derived mesenchymal stem cells ; BM-MSC ; bone marrow-derived mesenchymal stem cells ; SOMA ; SOMAscan assay ; a trademark by SomaLogic ; Boulder CO ; RNA ; RNA sequencing ; PROT ; liquid chromatography coupled to mass spectrometry-based proteomics ; SDEs ; significant differentially expressed
刊名：Journal of Proteomics
出版年：2017
出版时间：6 January 2017
年：2017
卷：150
期：Complete
页码：86-97
全文大小：2153 K

文摘

class="label">•: SOMAscan performance has been compared side by side to LC-MS/MS and RNA-seq analysis.
class="label">•: Complementarity of SOMAscan to LC-MS/MS and RNA-seq improves molecular characterization of MSC.
class="label">•: ESC derived MSC are potentially more suitable for treatment of neurological diseases.
class="label">•: In-depth exploration of ESC and MSC CD markers and signaling molecules by SOMAscan