Enhancement of a high efficient autoinducible expression system in Bacillus subtilis by promoter engineering

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Enhancement of a high efficient autoinducible expression system in Bacillus subtilis by promoter engineering

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作者：Jintao Cheng ; Chengran Guan ; Wenjing Cui ; Li Zhou ; Zhongmei Liu ; Weijiang Li ; ^{wjlee01@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Zhemin Zhou ; ^{zhmzhou@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author}
关键词：Promoter ; Expression system ; Autoinducible ; Overexpression
刊名：Protein Expression and Purification
出版年：2016
出版时间：November 2016
年：2016
卷：127
期：Complete
页码：81-87
全文大小：819 K

文摘

class="label">•: Promoter P_srfA was optimized to construct by engineering its length and the core regions.
class="label">•: An autoinducible expression system was constructed with the enhanced P_srfA derivative.
class="label">•: Heterologous proteins GFP, NK and AP could be overexpressed.

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