Guidelines for cloning, expression, purification and functional characterization of primary HIV-1 envelope glycoproteins

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Guidelines for cloning, expression, purification and functional characterization of primary HIV-1 envelope glycoproteins

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作者：Yann Benureau^a ; ^b ; ^{yann.benureau@free.fr" class="auth_mail" title="E-mail the corresponding author} ; Philippe Colin^a ; ^b ; ^{philippe.colin1@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Isabelle Staropoli^a ; ^b ; ^{isabelle.staropoli@pasteur.fr" class="auth_mail" title="E-mail the corresponding author} ; Nuria Gonzalez^c ; ^{nglez@isciii.es" class="auth_mail" title="E-mail the corresponding author} ; Javier Garcia-Perez^c ; ^{eoaz@isciii.es" class="auth_mail" title="E-mail the corresponding author} ; Jose Alcami^c ; ^{ppalcami@isciii.es" class="auth_mail" title="E-mail the corresponding author} ; Fernando Arenzana-Seisdedos^a ; ^b ; ^{farenzan@pasteur.fr" class="auth_mail" title="E-mail the corresponding author} ; Bernard Lagane^a ; ^b ; ^{bernard.lagane@pasteur.fr" class="auth_mail" title="E-mail the corresponding author}
关键词：HIV-1 gp120 ; AIDS ; Cloning ; Gene toxicity ; Protein expression and purification ; Receptor binding assays
刊名：Journal of Virological Methods
出版年：2016
出版时间：October 2016
年：2016
卷：236
期：Complete
页码：184-195
全文大小：2296 K

文摘

class="label">•: A reliable protocol for producing primary monomeric HIV-1 gp120 is presented.
class="label">•: The protocol avoids renowned problems in amplifying and cloning HIV-1 Env sequences.
class="label">•: The conditions for studying the gp120 interactions with CD4 and CCR5 are described.
class="label">•: The binding assay to CD4 is adaptable to large collections of glycoproteins.
class="label">•: The receptor binding assays confirmed that the gp120 are functionally relevant.

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