Microbial transformation of 20(S)-protopanaxadiol by Absidia corymbifera. Cytotoxic activity of the metabolites against human prostate cancer cells

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• 作者：Guangtong Chen^a ; Min Yang^b ; Shaojun Nong^a ; Xue Yang^a ; Yong Ling^a ; Donggeng Wang^a ; Xinyang Wang^a ; Wei Zhang^a ; ^{zwei@ntu.edu.cn}
• 关键词：Microbial transformation ; Absidia corymbifera AS 3.3387 ; 20(S)-Protopanaxadiol ; Dehydrogenation ; Hydroxylation ; Cytotoxic activity
• 刊名：Fitoterapia
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：84
• 期：Complete
• 页码：6-10
• 全文大小：248 K

文摘

Biotransformation of 20(S)-protopanaxadiol (1) by the fungus Absidia corymbifera AS 3.3387 yielded five metabolites (2-6). On the basis of spectroscopic data analyses, the metabolites were identified as 26-hydroxyl-20(S)-protopanaxadiol (2), 23, 24-en-25-hydroxyl-20(S)-protopanaxadiol (3), 25-hydroxyl-20(S)-protopanaxadiol (4), 7¦Â-hydroxyl-20(S)-protopanaxatriol (5), and 7-oxo-20(S)-protopanaxatriol (6), respectively. Among them, 5 and 6 are new compounds. These results indicated that A. corymbifera AS 3.3387 could catalyze the side-chain oxidation-reduction, 7¦Â hydroxylation, and the specific C-7 dehydrogenation of derivatives of 20(S)-protopanaxadiol. The metabolites 2, 5, and 6 showed the more potent inhibitory effects against DU-145 and PC-3 cell lines than the substrate.