A high-throughput, modified ALS activity assay for Cyperus difformis and Schoenoplectus mucronatus seedlings

详细信息    查看全文

• 作者：Rafael M. Pedroso ; Kassim Al-Khatib ; ^{kalkhatib@ucdavis.edu} ; Bradley D. Hanson ; Albert J. Fischer
• 关键词：ALS ; Acetolactate synthase ; PSII ; Photosystem II ; R ; Herbicide-resistant ; CYPDI ; Cyperus difformis ; SCHMU ; Schoenoplectus mucronatus ; I50 ; Herbicide rate that decreases ALS activity by 50%
• 刊名：Pesticide Biochemistry and Physiology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：135
• 期：Complete
• 页码：78-81
• 全文大小：364 K
• 卷排序：135

文摘

ALS activity of putative R CYPDI and SCHMU biotypes was not inhibited by bensulfuron-methyl. Elevated ALS resistance (I50 ≥ 10,000 in R) likely stems from an altered target-site. Results indicated R biotypes do not have an enhanced production of ALS enzymes. Cross-resistance to other herbicidal modes of action is unlikely in R CYPDI and SCHMU. The optimized ALS assay led to a 1.5 to 3-fold increase in number of assays per g tissue. New protocol can suit weeds that produce small amount of foliage at early growth stages.