Aptamer-functionalized silver nanoparticles for scanometric detection of platelet-derived growth factor-BB

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• 作者：Hongting Hu ; Hui Li ; Yaju Zhao ; Shiyu Dong ; Wei Li ; Weibing Qiang ; Danke Xu
• 关键词：Silver nanoparticles ; Gold nanoparticles ; Aptamer ; Catalysis ; Scanometric detection
• 刊名：Analytica Chimica Acta
• 出版年：17 February, 2014
• 年：2014
• 卷：812
• 期：Complete
• 页码：152-160
• 全文大小：2057 K

文摘

In this work, we reported a scanometric assay system based on the aptamer-functionalized silver nanoparticles (apt-AgNPs) for detection of platelet-derived growth factor-BB (PDGF-BB) protein. The aptamer and ssDNA were bound with silver nanoparticles by self-assembly of sulfhydryl group at 5鈥?end to form the apt-AgNPs probe. The apt-AgNPs probe can catalyze the reduction of metallic ions in color agent to generate metal deposition that can be captured both by human eyes and a flatbed scanner. Two different color agents, silver enhancer solution and color agent 1 (10 mM HAuCl₄ + 2 mM hydroquinone) were used to develop silver and gold shell on the surface of AgNPs separately. The results demonstrated that the formation of Ag core-Au shell structure had some advantages especially in the low concentrations. The apt-AgNPs probe coupled with color agent 1 showed remarkable superiority in both sensitivity and detection limit compared to the apt-AuNPs system. The apt-AgNPs system also produced a wider linear range from 1.56 ng mL^鈭? to 100 ng mL^鈭? for PDGF-BB with the detection limit lower than 1.56 ng mL^鈭?. The present strategy was applied to the determination of PDGF-BB in 10% serum, and the results showed that it had good specificity in complex biological media.