Supercritical fluid chromatography for separation and preparation of tautomeric 7-epimeric spiro oxindole alkaloids from Uncaria macrophylla

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• 作者：Wenzhi Yang^a ; ¹ ; Yibei Zhang^a ; ¹ ; Huiqin Pan^a ; ^b ; ¹ ; Changliang Yao^a ; ^b ; Jinjun Hou^a ; Shuai Yao^a ; Luying Cai^a ; Ruihong Feng^a ; Wanying Wu^a ; ^{wanyingwu@simm.ac.cn} ; Dean Guo^a
• 关键词：Spiro oxindole alkaloid ; Uncaria macrophylla ; Isomerization ; Ultra-performance convergence chromatography ; Preparative scale supercritical fluid chromatography
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：5 February 2017
• 年：2017
• 卷：134
• 期：Complete
• 页码：352-360
• 全文大小：2759 K
• 卷排序：134

文摘

SFC was used to separate and isolate two pairs of 7-epimers of SOAs. Acetonitrile stabilized two pairs of epimers and was used as modifier in SFC. Two achiral UPC2 methods were established on the Torus 1-AA and Diol columns. Preparative SFC enabled isolation of four SOA compounds with the purity >95%. SFC provides a solution to preparation of high-purity reference standards.