In Sj?gren's syndrome, B lymphocytes induce epithelial cells of salivary glands into apoptosis through protein kinase C delta activation

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• 作者：Marie-Michè ; le Varin ; Thomas Guerrier ; Valé ; rie Devauchelle-Pensec ; Christophe Jamin ; Pierre Youinou ; ^{youinou@univ-brest.fr} ; Jacques-Olivier Pers
• 关键词：Sjö ; gren's syndrome ; B lymphocyte ; Apoptosis ; Epithelial cells
• 刊名：Autoimmunity Reviews
• 出版年：2012
• 出版时间：February 2012
• 年：2012
• 卷：11
• 期：4
• 页码：252-258
• 全文大小：835 K

文摘

Sj?gren's syndrome (SS) is a chronic autoimmune epithelitis associated with diffuse lymphocytic infiltration that varies in composition and differs according to lesion severity. T lymphocytes have been viewed as competent in their own right in the destruction of epithelial cells, whereas B lymphocytes that predominate in severe lesions have never been implicated in direct tissue damage. Using co-culture experiments with human salivary gland (HSG) cell line cells and tonsilar B lymphocytes, we observed that direct HSG cell-B lymphocyte contacts were able to induce apoptosis in epithelial cells. This B lymphocyte-mediated cell death could not be ascribed to Fas-Fas ligand interactions but required translocation of protein kinase C delta (PKC ¦Ä) into the nucleus of epithelial cells. Ultimately, activation of PKC¦Ä resulted in histone H2B phosphorylation on serine 14 and poly (ADP-ribose) polymerase cleavage. Our results suggest that B lymphocytes infiltrating the SGs of patients with SS could evoke epithelial cell apoptosis.