NMR based structural studies decipher stacking of the alkaloid coralyne to terminal guanines at two different sites in parallel G-quadruplex DNA, [d(TTGGGGT)]₄ and [d(TTAGGGT)]₄

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• 作者：Kumar Padmapriya ; Ritu Barthwal ; ^{ritubfbs@iitr.ac.in}
• 关键词：Telomeric G-quadruplex DNA ; Alkaloid coralyne ; Circular dichroism ; Nuclear magnetic resonance ; Thermal stabilization ; Restrained molecular dynamics
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1861
• 期：2
• 页码：37-48
• 全文大小：2814 K
• 卷排序：1861

文摘

First NMR based solution structure of 2:1 coralyne-[d(TTGGGGT)]4 complex. Coralyne stacks at T2pG3/A3pG4 and G6pT7 steps in [d(TTGGGGT)]4/ [d(TTAGGGT)]4. Binding leads to thermal stabilization of [d(TTGGGGT)]4/[d(TTAGGGT)]4 by 25–35 °C. Coralyne induces switch in anti-parallel 22-mer G-quadruplex DNA to parallel form. Induced negative CD band observed at 300–315 nm in 7-mer G-quadruplex DNA.