Exploring the potential of genome editing CRISPR-Cas9 technology

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• 作者：Vijai Singh^a ; ^{vijaisingh15@gmail.com} ; ^{vijai.singh@issb.genopole.fr} ; Darren Braddick^a ; Pawan Kumar Dhar^b
• 关键词：Cas ; CRISPR-associated proteins ; cccDNA ; covalently closed circular DNA ; CCR5 ; C-C chemokine receptor type 5 ; CHO ; Chinese hamster ovary ; CRISPRs ; clustered regularly interspaced short palindromic repeats ; CRISPRa ; CRISPR activation ; CRISPRi ; CRISPR interference ; crRNAs ; CRISPR RNAs ; dCas9 ; dead Cas9 ; DMD ; Duchenne muscular dystrophy ; DSB ; double-strand break ; eGFP ; enhanced green fluorescent protein ; HDR ; homology-directed repair ; HEK 293 ; human embryonic kidney 293 ; Indel ; insertion and deleti
• 刊名：Gene
• 出版年：2017
• 出版时间：30 January 2017
• 年：2017
• 卷：599
• 期：Complete
• 页码：1-18
• 全文大小：1154 K
• 卷排序：599

文摘

CRISPR-Cas is an RNA-guided adaptive immune system of bacteria that protects from phages and plasmids. Type II CRISPR-Cas9 has recently emerged as a key technology for targeted genome editing of many organisms. It has been used to create animal models, control infection of bacteria and viruses, and correct defective genes. Repurposing CRISPR-Cas9 has been used for targeted gene repression, activation, and loci imaging. CRISPR-Cas9 shows great potential for biomedical, therapeutic, industrial and biotechnological applications.