Rapid kinetic methods to dissect steroidogenic cytochrome P450 reaction mechanisms

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Rapid kinetic methods to dissect steroidogenic cytochrome P450 reaction mechanisms

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作者：Francis K. Yoshimoto^a ; Richard J. Auchus^b ; ^c ; ^{rauchus@med.umich.edu" class="auth_mail" title="E-mail the corresponding author}
关键词：CYP ; cytochrome P450 ; POR ; P450 oxidoreductase (cytochrome P450 reductase) ; cDNA ; complementary DNA ; FDX1 ; ferredoxin ; FDXR ; ferredoxin reductase ; EPR ; electron paramagnetic resonance ; KIE ; kinetic isotope effect ; UV&ndash ; vis ; ultraviolet&ndash ; visible
刊名：Journal of Steroid Biochemistry and Molecular Biology
出版年：2016
出版时间：July 2016
年：2016
卷：161
期：Complete
页码：13-23
全文大小：1938 K

文摘

•: The cytochrome P450 reaction cycle has several microscopic steps.
•: Rapid-kinetics experiments permit the study of transient intermediates.
•: Single-turnover experiments determine if product release is rate-limiting.
•: A kinetic isotope effect is observed if CH bond breaking is rate-limiting.

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