The establishment of Saccharomyces boulardii surface display system using a single expression vector

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• 作者：Tiantian Wang ; Hui Sun ; Jie Zhang ; Qing Liu ; Longjiang Wang ; Peipei Chen ; Fangkun Wang ; Hongmei Li ; Yihong Xiao ; Xiaomin Zhao
• 关键词：S. boulardii ; Surface display ; a-Agglutinin ; EGFP ; EtMic2p
• 刊名：Fungal Genetics and Biology
• 出版年：March, 2014
• 年：2014
• 卷：64
• 期：Complete
• 页码：1-10
• 全文大小：1551 K

文摘

In the present study, an a-agglutinin-based Saccharomyces boulardii surface display system was successfully established using a single expression vector. Based on the two protein co-expression vector pSP-G1 built by Partow et al., a S. boulardii surface display vector-pSDSb containing all the display elements was constructed. The display results of heterologous proteins were confirmed by successfully displaying enhanced green fluorescent protein (EGFP) and chicken Eimeria tenella Microneme-2 proteins (EtMic2) on the S. boulardii cell surface. The DNA sequence of AGA1 gene from S. boulardii (SbAGA1) was determined and used as the cell wall anchor partner. This is the first time heterologous proteins have been displayed on the cell surface of S. boulardii. Because S. boulardii is probiotic and eukaryotic, its surface display system would be very valuable, particularly in the development of a live vaccine against various pathogenic organisms especially eukaryotic pathogens such as protistan parasites.