DNAzyme-based biosensor for Cu<sup>2+</sup> ion by combining hybridization chain reaction with fluorescence resonance energy transfer technique

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DNAzyme-based biosensor for Cu²⁺ ion by combining hybridization chain reaction with fluorescence resonance energy transfer technique

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作者：Ying Chen^a ; Ling Chen^a ; Yidian Ou^a ; Zhenhua Wang^b ; ^{wzh312@mail.sdu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Fengfu Fu^a ; Liangqia Guo^a ; ^{lqguo@fzu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
关键词：Cu2+ ion ; DNAzyme ; Hybridization chain reaction ; Fluorescence resonance energy transfer ; Ratiometric measurement ; Magnetic beads
刊名：Talanta
出版年：2016
出版时间：1 August 2016
年：2016
卷：155
期：Complete
页码：245-249
全文大小：630 K

文摘

•: A signal amplification strategy was developed for Cu²⁺ by combining HCR with FRET.
•: Cu²⁺-dependent DNAzyme enhanced the specificity of the biosensor for Cu²⁺ ion.
•: The cleaved substrate strands were used as the initiator for HCR.
•: Ratiometric measurement was used to detect Cu²⁺ ion with a LOD of 0.5 nmol L^-1. .

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