Greater filamin C, GSK3¦Á, and GSK3¦Â serine phosphorylation in insulin-stimulated isolated skeletal muscles of calorie restricted 24 month-old rats

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• 作者：Donel A. Sequea^a ; Naveen Sharma^b ; ^{naveens@umich.edu} ; Edward B. Arias^b ; Gregory D. Cartee^a ; ^b ; ^c ; ^{gcartee@umich.edu}
• 关键词：Ageing ; Insulin signaling ; Muscle fiber type ; Dietary restriction
• 刊名：Mechanisms of Ageing and Development
• 出版年：2013
• 出版时间：January-February, 2013
• 年：2013
• 卷：134
• 期：1-2
• 页码：60-63
• 全文大小：493 K

文摘

Moderate calorie restriction (CR) can improve insulin-stimulated Akt phosphorylation and glucose uptake in muscles from 24 month-old rats, but the specific Akt substrates linking CR-effects on Akt to glucose uptake and other cellular processes are uncertain. We probed CR's influence on site-specific phosphorylation of five Akt substrates (AS160^Ser588, TBC1D1^Thr596, FLNc^Ser2213, GSK3¦Á^Ser21, and GSK3¦Â^Ser9) in predominantly fast-twitch (epitrochlearis) and predominantly slow-twitch (soleus) muscles. We observed no CR-effect on phosphorylation of AS160^Ser588 or TBC1D1^Thr596, but there was a CR-induced increase in insulin-stimulated FLNc^Ser2213, GSK3¦Á^Ser21, and GSK3¦Â^Ser9 phosphorylation for both muscles. These results indicate that CR does not uniformly affect insulin-mediated phosphorylation of Akt substrates in fast- or slow-twitch muscles from 24 month-old rats.