Systematic time-dependent visualization and quantitation of the neurogenic rate in brain organoids

详细信息    查看全文

• 作者：Yoichi Kosodo^a ; ^b ; ^{kosodo@kbri.re.kr} ; Taeko Suetsugu^a ; Tetsuya J. Kobayashi^c ; Fumio Matsuzaki^a
• 关键词：Quantitative image processing ; Tbr2 ; Corticogenesis ; Microfabrication ; Neural ball ; DAPT
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：29 January 2017
• 年：2017
• 卷：483
• 期：1
• 页码：94-100
• 全文大小：2159 K
• 卷排序：483

文摘

We established a semi-automatic neurogenic activity assay system in brain organoids. Microcavity chips and image processing enabled the accurate scoring of fluorescence. The temporal response to a drug that potentiates neurogenesis was demonstrated.