Detection of high-risk human papillomavirus infection in tonsillar specimens using 2 commercially available assays

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• 作者：Cara C. Cockerill^a ; ^{Cockerill.cara@mayo.edu" class="auth_mail" title="E-mail the corresponding author} ; Laura J. Orvidas^a ; ^{Orvidas.laura@mayo.edu" class="auth_mail" title="E-mail the corresponding author} ; Eric J. Moore^a ; ^{Moore.eric@mayo.edu" class="auth_mail" title="E-mail the corresponding author} ; Matthew J. Binnicker^b ; ^{Binnicker.Matthew@mayo.edu" class="auth_mail" title="E-mail the corresponding author} ; Brian J. Duresko^b ; ^{Duresko.brian@mayo.edu" class="auth_mail" title="E-mail the corresponding author} ; Mark J. Espy^b ; ^{Espy.mark@mayo.edu" class="auth_mail" title="E-mail the corresponding author} ; Franklin R. Cockerill^b ; ¹ ; ^{Franklincockerill@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Nicole M. Tombers^a ; ^{Tombers.nicole@mayo.edu" class="auth_mail" title="E-mail the corresponding author} ; Bobbi S. Pritt^b ; ^{Pritt.Bobbi@mayo.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Tonsil ; Human papillomavirus ; Oropharyngeal cancer
• 刊名：Diagnostic Microbiology & Infectious Disease
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：86
• 期：4
• 页码：365-368
• 全文大小：207 K

文摘

Patients undergoing tonsillectomy for nonmalignant causes were enrolled. A flocked swab and fresh tissue were collected from the left and right tonsil of each patient. Specimens were tested for hrHPV DNA using the Roche cobas test and for the presence of E6/E7 messenger RNA using the Hologic Aptima hrHPV test.

Of the 193 patients enrolled, 129 were in the pediatric group (ages 1–12 years; median, 5 years), and 64 were in the adult group (ages 13–55; median, 22 years). All swab and tissue specimens were negative for hrHPV by both methods. Positive, negative, and internal controls performed as expected.

We found a 0% rate of infection indicating that detectable hrHPV infection in tonsillar tissue appears to be uncommon in the children and adults in the population sampled.