Derivation of FSHD1 affected human embryonic stem cell line Genea049

详细信息    查看全文

• 作者：Biljana Dumevska ; ¹ ; ^{biljana.dumevska@geneabiocells.com" class="auth_mail" title="E-mail the corresponding author} ; Omar Chami¹ ; Robert McKernan ; Divya Goel ; Uli Schmidt
• 刊名：Stem Cell Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：16
• 期：2
• 页码：469-471
• 全文大小：589 K

文摘

The Genea049 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying a deletion in 4q35 with only 5 D4Z4 repeats by PGD linkage analysis, indicative of FSHD1. Following ICM outgrowth on inactivated human feeders, karyotype was confirmed as 46, XX by CGH and STR analysis demonstrated a female Allele pattern. The hESC line had pluripotent cell morphology, 90% of cells expressed Nanog, 96% Oct4, 80% Tra1–60 and 99% SSEA4, gave a Pluritest Pluripotency score of 23.16, Novelty of 1.43 and demonstrated Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and visible contamination.