Serodiagnosis of bovine trypanosomosis caused by non-tsetse transmitted Trypanosoma (Duttonella) vivax parasites using the soluble form of a Trypanozoon variant surface glycoprotein antigen

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Serodiagnosis of bovine trypanosomosis caused by non-tsetse transmitted Trypanosoma (Duttonella) vivax parasites using the soluble form of a Trypanozoon variant surface glycoprotein antigen

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作者：Graciela L. Uzcanga^a ; ^b ; ^c ; Yenis Pé ; rez-Rojas^a ; Roc&iacute ; o Camargo^a ; Adriana Izquier^a ; José ; A. Noda^a ; ^d ; Ronny Chac&iacute ; n^a ; ^e ; Nereida Parra^a ; ^f ; ^g ; Lenin Ron^c ; ^h ; Richar Rodr&iacute ; guez-Hidalgo^c ; ⁱ ; José ; Bubis^a ; ^{jbubis@usb.ve" class="auth_mail" title="E-mail the corresponding author}
关键词：VSG ; variant surface glycoprotein ; VAT ; variant antigen type ; RoTat ; RodeTrypanozoon antigen type ; ELISA ; enzyme-linked immunosorbent assay ; MHC ; microhematocrit centrifuge ; PCR ; polymerase chain reaction ; ITS1 ; internal transcribed spacer 1 region ; MPP ; microparticles ; ABTS ; 2 ; 2&prime ; -azino-bis (3-ethylbenzthiazoline-6-sulfonic acid ; PCV ; packed cell volume ; HAT ; human african trypanosomiasis ; DME ; direct microscopic examination ; IIF ; indirect immunofluorescence
刊名：Veterinary Parasitology
出版年：2016
出版时间：15 March 2016
年：2016
卷：218
期：Complete
页码：31-42
全文大小：1484 K

文摘

•: p64 from T. equiperdum is a potent diagnostic tool for acute bovine trypanosomosis in Latin America.
•: T. equiperdum p64 is a potent reagent to diagnose chronic bovine trypanosomosis in Latin America.
•: A significant agreement was seen between assays using p64 and RoTat 1.2 VSG.
•: A successful agglutination test was designed using p64 coupled to latex particles.