Synthesis of propyl-β-d-galactoside with free and immobilized β-galactosidase from Aspergillus oryzae

详细信息    查看全文

• 作者：Carlos Vera ; ^{carlos.vera@pucv.cl} ; Cecilia Guerrero ; Lorena Wilson ; André ; s Illanes
• 关键词：2-Nitrophenyl-β-d-galactopyranoside (PubChem CID ; 96647) ; α-Lactose (PubChem CID ; 84571) ; 1-Propanol (PubChem CID ; 1031)
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：53
• 期：Complete
• 页码：162-171
• 全文大小：828 K
• 卷排序：53

文摘

Synthesis of propyl-β-d-galactoside with β-galactosidase from A. oryzae was optimized by RSM. A. oryzae β-galactosidase was immobilized in agarose supports and by crosslinking and aggregation. Highest yield (0.87 mol/mol) was obtained with the enzyme immobilized in glyoxyl-agarose. Cumulative productivity of the glyoxyl-agarose derivative doubled that of the free enzyme. An enzymatic procedure to obtain pure propyl-β-d-galactoside has been developed.