MiR-150 Controls B Cell Differentiation by Targeting the Transcription Factor c-Myb

详细信息    查看全文

• 作者：Changchun Xiao ; Dinis Pedro Calado ; Gunther Galler ; To-Ha Thai ; Heide Christine Patterson ; Jing Wang ; Nikolaus Rajewsky ; Timothy P. Bender ; Klaus Rajewsky
• 关键词：MOLIMMUNO ; RNA
• 刊名：Cell
• 出版年：2007
• 出版时间：5 October 2007
• 年：2007
• 卷：131
• 期：1
• 页码：146-159
• 全文大小：2031 K

文摘

MiR-150 is a microRNA (miRNA) specifically expressed in mature lymphocytes, but not their progenitors. A top predicted target of miR-150 is c-Myb, a transcription factor controlling multiple steps of lymphocyte development. Combining loss- and gain-of-function gene targeting approaches for miR-150 with conditional and partial ablation of c-Myb, we show that miR-150 indeed controls c-Myb expression in vivo in a dose-dependent manner over a narrow range of miRNA and c-Myb concentrations and that this dramatically affects lymphocyte development and response. Our results identify a key transcription factor as a critical target of a stage-specifically expressed miRNA in lymphocytes and suggest that this and perhaps other miRNAs have evolved to control the expression of just a few critical target proteins in particular cellular contexts.