Development and evaluation of a novel RT-qPCR based test for the quantification of HER2 gene expression in breast cancer

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• 作者：Hicham El Hadi^a ; ^b ; ^{h.elhadi@mascir.com} ; Imane Abdellaoui-Maane^a ; ^b ; ^{i.abdellaoui@mascir.com} ; Denise Kottwitz^a ; ^{denisekottwitz@web.de} ; Manal El Amrani^a ; ^{dahmani_manale@yahoo.fr} ; Nadia Bouchoutrouch^a ; ^{n.bouchoutrouch@mascir.com} ; Zineb Qmichou^a ; ^{z.qmichou@mascir.com} ; Mehdi Karkouri^c ; ^{mehdi.karkouri@menara.ma} ; Hicham ElAttar^d ; Hassan Errihani^e ; Pedro L Fernandez^f ; ^{plfernan@clinic.ub.es} ; Youssef Bakri^b ; ^{ybakri@gmail.com} ; Hassan Sefrioui^a ; ^{h.sefrioui@mascir.com} ; Abdeladim Moumen^a ; ^{a.moumen@mascir.com}
• 关键词：RT-qPCR ; reverse transcription quantitative reel time polymerase chain reaction ; HER2 ; human epidermal growth factor receptor 2 ; GAPDH ; glucophosphate dehydrogenase ; PPIA ; peptidylprolyl isomerase A ; RPL37a ; Ribosomal protein L37-alpha ; RPL30 ; Ribosomal protein L30 ; MRPL19 ; mitochondrial ribosomal protein L19 ; PUM1 ; pumilio RNA-binding family member 1 ; UBB ; ubiquitin B ; IHC ; immunohistochemistry ; FISH ; fluorescence in situ hybridization ; ECG ; endogenous control gene
• 刊名：Gene
• 出版年：2017
• 出版时间：20 March 2017
• 年：2017
• 卷：605
• 期：Complete
• 页码：114-122
• 全文大小：706 K
• 卷排序：605

文摘

Analysis of HER2 status in breast cancer samples Use of qPCR in the analysis of HER2 expression in breast cancer Identification of the most stable control gene for an accurate analysis of HER2 expression in biopsy samples Clinical validation of the RT-qPCR based HER2 quantification test