Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics

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• 作者：Jennifer M. Spaethling¹ ; ⁵ ; Young-Ji Na² ; ⁵ ; Jaehee Lee¹ ; ⁵ ; Alexandra V. Ulyanova³ ; Gordon H. Baltuch³ ; Thomas J. Bell¹ ; Steven Brem³ ; H. Isaac Chen³ ; Hannah Dueck² ; Stephen A. Fisher² ; Marcela P. Garcia¹ ; Mugdha Khaladkar² ; David K. Kung³ ; Timothy H. Lucas Jr.³ ; Donald M. O&rsquo ; Rourke³ ; Derek Stefanik² ; Jinhui Wang¹ ; John A. Wolf³ ; Tamas Bartfai⁴ ; M. Sean Grady³ ; Jai-Yoon Sul¹ ; Junhyong Kim² ; James H. Eberwine¹ ; ⁶ ; ^{eberwine@upenn.edu}
• 关键词：single cell ; human ; primary cell ; adult ; aged ; neuron ; astrocyte ; microglia ; endothelial cell ; transcriptome
• 刊名：Cell Reports
• 出版年：2017
• 出版时间：17 January 2017
• 年：2017
• 卷：18
• 期：3
• 页码：791-803
• 全文大小：4040 K
• 卷排序：18

文摘

A system for culturing adult human neuronal cells after neurosurgery is presented Among the many adult human brain cells that are long-term cultured are neurons Human brain cell-type-enriched pri-miRNAs and lncRNAs are characterized Cell-type- and patient-specific transcriptional hierarchies were revealed