Sweetpotato vines hydrolysate induces glycerol to be an effective substrate for lipid production of Trichosporon fermentans

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• 作者：Qi Shen^b ; ¹ ; Hui Lin^a ; ¹ ; Jumei Zhan^b ; Qun Wang^a ; Yuhua Zhao^a ; ^{yhzhao225@zju.edu.cn}
• 关键词：Glycerol ; Sweetpotato vines ; Enzymatic hydrolysate ; Trichosporon fermentans ; Single cell oils (SCOs)
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：136
• 期：Complete
• 页码：725-729
• 全文大小：521 K

文摘

By co-fermented with sweetpotato vines hydrolysate (SVH), glycerol can be used as an effective substrate for Trichosporon fermentans to produce lipids. Submerged fermentation results showed that T. fermentans exhibited a maximum lipid yield of 7.45 g l^?1 with a biomass of 17.95 g l^?1 on 10 % SVH added glycerol mineral medium (Glycerol MM-10 % SVH), which was 4.34-fold higher than that on glycerol mineral medium. Lipids produced on glycerol based media exhibited a significantly different fatty acid composition profile from that produced on sugar based media accompanying by a sharp increase in polyunsaturated fatty acids content. Biochemical behaviors characterization further demonstrated that SVH has a remarkable promoting effect on the biomass formation and glycerol uptake. The extremely high lipid yield on Glycerol MM-10 % SVH was mainly attributed to the enhancement of SVH on biomass, although SVH is an excellent nutrient supplier for lipid accumulation due to its low nitrogen availability.