A Multi-step Transcriptional and Chromatin State Cascade Underlies Motor Neuron Programming from Embryonic Stem Cells

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• 作者：Silvia Velasco¹ ; ⁷ ; Mahmoud M. Ibrahim² ; ³ ; ⁷ ; Akshay Kakumanu⁴ ; ⁷ ; G&ouml ; rkem Garipler¹ ; Begü ; m Aydin¹ ; Mohamed Ahmed Al-Sayegh¹ ; ⁵ ; Antje Hirsekorn³ ; Farah Abdul-Rahman¹ ; Rahul Satija¹ ; ⁶ ; Uwe Ohler² ; ³ ; ^{uwe.ohler@mdc-berlin.de} ; Shaun Mahony⁴ ; ^{mahony@psu.edu} ; Esteban O. Mazzoni¹ ; ⁸ ; ^{eom204@nyu.edu}
• 关键词：direct programming ; neuron ; motor neuron
• 刊名：Cell Stem Cell
• 出版年：2017
• 出版时间：2 February 2017
• 年：2017
• 卷：20
• 期：2
• 页码：205-217.e8
• 全文大小：3379 K
• 卷排序：20

文摘

ESC expression of Ngn2/Isl1/Lhx3 induces rapid transcriptional and chromatin changes At early stages, Isl1/Lhx3 (homeodomain) and Ngn2 (bHLH) target distinct genomic sites As programming progresses, Isl1/Lhx3 binding shows dynamic relocalization Ngn2-induced factors guide Isl1/Lhx3 redistribution to initially inaccessible sites