Induced Quiescence of Lgr5+ Stem Cells in Intestinal Organoids Enables Differentiation of Hormone-Producing Enteroendocrine Cells

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• 作者：Onur Basak¹ ; ² ; ⁵ ; Joep Beumer¹ ; ² ; ⁵ ; Kay Wiebrands¹ ; ² ; ⁵ ; Hiroshi Seno⁴ ; Alexander van Oudenaarden¹ ; ² ; Hans Clevers¹ ; ² ; ³ ; ⁶ ; ^{h.clevers@hubrecht.eu}
• 关键词：organoids ; quiescence ; intestinal stem cells ; EGFR signaling ; enteroendocrine cell ; Lgr5
• 刊名：Cell Stem Cell
• 出版年：2017
• 出版时间：2 February 2017
• 年：2017
• 卷：20
• 期：2
• 页码：177-190.e4
• 全文大小：4712 K
• 卷排序：20

文摘

EGFR inhibition halts DNA replication and proliferation of Lgr5+ ISCs through MEK Lgr5+ ISCs reactivated from quiescence retain multilineage differentiation potential Combined EGFR/Wnt/Notch inhibition produces enteroendocrine cells with high purity RNA sequencing shows regional identity and heterogeneity in hormone-producing EECs