Structural characterization of the α-mating factor prepro-peptide for secretion of recombinant proteins in Pichia pastoris

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• 作者：Sabreen Chahal^a ; ¹ ; Peter Wei^a ; ¹ ; Pachai Moua^a ; Sung Pil James Park^a ; Janet Kwon^a ; Arth Patel^a ; Anthony T. Vu^a ; Jason A. Catolico^a ; Yu Fang Tina Tsai^a ; Nadia Shaheen^a ; Tiffany T. Chu^a ; Vivian Tam^a ; Zill-E-Huma Khan^a ; Hyun Henry Joo^b ; Liang Xue^b ; Joan Lin-Cereghino^a ; Jerry W. Tsai^b ; Geoff P. Lin-Cereghino^a ; ² ; ^{glincere@pacific.edu}
• 关键词：Pichia pastoris ; Recombinant expression ; Secretion leader ; Alpha mating factor ; Circular dichroism ; Protein modeling ; Knob socket
• 刊名：Gene
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：598
• 期：Complete
• 页码：50-62
• 全文大小：1409 K
• 卷排序：598

文摘

Mutations were made in the MATα prepro leader to determine their effect on secretion. Circular dichroism showed changes in secondary structure of the wild type vs. mutant pro-peptides. CD results led to a model of the MATα pro-peptide different from that created by bioinformatics. The model emphasizes the importance of the N and C termini in pro-peptide function.