Magnetic nanoparticles coated with maltose-functionalized polyethyleneimine for highly efficient enrichment of N-glycopeptides

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Magnetic nanoparticles coated with maltose-functionalized polyethyleneimine for highly efficient enrichment of N-glycopeptides

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作者：Jinan Li^a ; ^b ; Fangjun Wang^a ; ^{wangfj@dicp.ac.cn" class="auth_mail" title="E-mail the corresponding author} ; Hao Wan^a ; ^b ; Jing Liu^a ; ^b ; Zheyi Liu^a ; ^b ; Kai Cheng^a ; ^b ; Hanfa Zou^a ; ^{hanfazou@dicp.ac.cn" class="auth_mail" title="E-mail the corresponding author}
关键词：N-glycopeptides enrichment ; Magnetic nanoparticles ; Polyethyleneimine ; Click chemistry ; Hydrophilic interaction chromatography
刊名：Journal of Chromatography A
出版年：2015
出版时间：18 December 2015
年：2015
卷：1425
期：Complete
页码：213-220
全文大小：1389 K

文摘

•: A novel adsorbent coated with branched PEI and maltose was prepared.
•: The adsorbent exhibited high hydrophilicity and selectivity.
•: The adsorbent exhibited high N-glycopeptide enrichment capacity (>150 mg/g, IgG/MNPs).
•: The adsorbent exhibited high N-glycopeptide enrichment recovery (85%).
•: 1567 N-glycopeptides were enriched from the complex protein sample.

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