A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors

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• 作者：Donald M. Bryant¹ ; ⁶ ; Kimberly Johnson¹ ; ⁶ ; Tia DiTommaso¹ ; Timothy Tickle² ; Matthew Brian Couger³ ; Duygu Payzin-Dogru¹ ; Tae J. Lee¹ ; Nicholas D. Leigh¹ ; Tzu-Hsing Kuo¹ ; Francis G. Davis¹ ; Joel Bateman¹ ; Sevara Bryant¹ ; Anna R. Guzikowski¹ ; Stephanie L. Tsai⁴ ; Steven Coyne¹ ; William W. Ye¹ ; Robert M. Freeman Jr.⁵ ; Leonid Peshkin⁵ ; Clifford J. Tabin⁴ ; Aviv Regev² ; Brian J. Haas² ; ^{bhaas@broadinstitute.org} ; Jessica L. Whited¹ ; ⁷ ; ^{jwhited@bwh.harvard.edu}
• 关键词：axolotl ; transcriptome ; Trinity ; Trinotate ; limb ; regeneration ; blastema ; expression analysis ; cirbp ; kazald1
• 刊名：Cell Reports
• 出版年：2017
• 出版时间：17 January 2017
• 年：2017
• 卷：18
• 期：3
• 页码：762-776
• 全文大小：5297 K
• 卷排序：18

文摘

Creation of a transcriptome with near-complete sequence data for 88% of axolotl genes Expression analyses identify tissue-enriched transcripts for key tissues The RNA-binding protein cirbp plays a cytoprotective role in limb regeneration Knockdown and overexpression of kazald1 in blastema cells impair limb regeneration