Knockdown of nuclease activity in the gut enhances RNAi efficiency in the Colorado potato beetle, Leptinotarsa decemlineata, but not in the desert locust, Schistocerca gregaria

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• 作者：Jornt Spit^a ; ^b ; ^{Jornt.spit@kuleuven.be} ; Annelies Philips^b ; Niels Wynant^a ; Dulce Santos^a ; Geert Plaetinck^b ; Jozef Vanden Broeck^a
• 关键词：RNA interference ; RNAi ; Colorado potato beetle ; Desert locust ; dsRNase ; Nuclease ; dsRNA
• 刊名：Insect Biochemistry and Molecular Biology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：81
• 期：Complete
• 页码：103-116
• 全文大小：3137 K
• 卷排序：81

文摘

Non-specific DNA/RNA endonucleases or dsRNases are expressed and secreted in the digestive system of many insect species. Degradation of dsRNA by nucleases in the digestive system of insects contributes to a lower environmental RNAi efficiency. Also the highly environmental RNAi sensitive Colorado potato beetle possesses two dsRNases active in the gut lumen. Knockdown of the dsRNases in the gut of the Colorado potato beetle increases the sensitivity towards orally delivered dsRNA. A similar strategy in the desert locust proved unsuccessful, probably due to residual nuclease activity.