Ethanol production from non-pretreated napiergrass through a simultaneous saccharification and fermentation process followed by a pentose fermentation with Escherichia coli KO11

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• 作者：Masahide Yasuda ; Akiteru Miura ; Tsutomu Shiragami ; Jin Matsumoto ; Ichiro Kamei ; Yasuyuki Ishii ; Kazuyoshi Ohta
• 关键词：Bioethanol ; Napiergrass ; Simultaneous saccharification-fermentation ; Cellulase from Acremonium ; Saccharomyces cerevisiae ; Escherichia coli KO11
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2012
• 出版时间：August, 2012
• 年：2012
• 卷：114
• 期：2
• 页码：188-192
• 全文大小：281 K

文摘

Efficient ethanol production from lignocellulosic napiergrass (Pennisetum purpureum Schumach) was examined by the combination of the simultaneous saccharification and fermentation (SSF) with commercial cellulase and Saccharomyces cerevisiae NBRC 2044 and subsequent pentose fermentation (PF) by Escherichia coli KO11. Under the optimized conditions, the combination of the SSF and PF processes resulted in the production of 144?mg?g^?1 of ethanol from the non-pretreated napiergrass powder. The ethanol yield was 44.2 % of the theoretical yield based on the hexose (37.5?g) and pentose (26.5?g) derived from 100?g of dry powdered napiergrass.