Catalytic, kinetic and thermodynamic properties of stabilized Bacillus stearothermophilus alkaline protease

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• 作者：Mohamed A. Abdel-Naby^a ; ^{mabdel_2010@yahoo.com} ; Samia A. Ahmed^a ; Hala R. Wehaidy^a ; Said A. El-Mahdy^b
• 关键词：Alkaline protease ; Stabilization ; Kinetic ; Thermodynamic properties
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：265-271
• 全文大小：938 K
• 卷排序：96

文摘

Evaluation of the stability of conjugated protease with several polysaccharides. Enhanced thermal and pH stability of protease on conjugation with pectin. Conjugate showed lower inactivation rate constant with increased half life. Conjugate protease showed lower values of ΔH*, ΔG*, ΔS*, ΔG*E−S, and ΔG*T−S. Conjugation showed significant impact on improvement of kcat, kcat/Km values.