Kinetic turbidimetry of patchy colloids aggregation: Latex particles immunoagglutination

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• 作者：Alexey A. Polshchitsin^a ; ^c ; Vyacheslav M. Nekrasov^a ; ^b ; Valentin S. Zakovryashin^c ; Galina E. Yakovleva^c ; Valeri P. Maltsev^a ; ^b ; ^d ; Maxim A. Yurkin^a ; ^b ; Andrei V. Chernyshev^a ; ^b ; ^{chern@ns.kinetics.nsc.ru}
• 关键词：Patchy particles ; Colloid aggregation kinetics ; Latex immunoagglutination ; Turbidimetric assay ; Fractal clusters ; Rate constant ; Biospecific binding ; Monte-Carlo simulation ; Gillespie algorithm
• 刊名：Colloids and Surfaces A: Physicochemical and Engineering Aspects
• 出版年：2017
• 出版时间：5 March 2017
• 年：2017
• 卷：516
• 期：Complete
• 页码：72-79
• 全文大小：868 K
• 卷排序：516

文摘

A new analytical expression for the treatment of the kinetic turbidimetry of patchy colloids aggregation was derived and verified. Diffusion-limited aggregation rate constants of chemically-anisotropic reactants with several small active sites were presented. Light scattering cross sections of the aggregates (of fractal structure) were calculated using the superposition T-matrix method. Gillespie method was applied to calculate stochastic kinetics of the aggregation (which is essential at low number of active sites on particles). A significant effect of the low number of active sites per particle on the turbimetric kinetics was found.