文摘
Motility of malignant cells plays a crucial role for the metastasis of tumours. Both, 17-b2;-estradiol and transforming growth factor-b2; (TGF-b2;), induce migration of MCF-7 breast cancer cells and simultaneous treatment resulted in an additive effect of the migratory response. But most interestingly, when cells were preincubated with 17-b2;-estradiol, the ability of TGF-b2; to evoke chemotaxis was drastically diminished. Abrogation of Smad signalling indicated that this pathway is essential for TGF-b2;-mediated MCF-7 cell migration. In agreement, pretreatment of MCF-7 cells with 17-b2;-estradiol resulted in a reduced phosphorylation of Smad2 and Smad3 as well as a diminished Smad2 and Smad3 gene reporter activity in response to TGF-b2;. Thus, these results indicate a controversial role of 17-b2;-estradiol on MCF-7 cell migration. 17-b2;-estradiol potently increases the migratory potency of MCF-7 cells, but inhibits TGF-b2;-induced migration by an interaction between estrogen receptors and Smad proteins.
