Stratum corneum targeting by dendritic core-multishell-nanocarriers in a mouse model of psoriasis

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• 作者：Hannah Pischon ; DVM^a ; ¹ ; Moritz Radbruch ; DVM^a ; Anja Ostrowski ; DVM ; PhD^a ; Pierre Volz ; MSc^b ; Christian Gerecke ; PhD^c ; Michael Unbehauen ; Dipl.-NanoSc^d ; Stefan Hö ; nzke ; RPh^e ; Sarah Hedtrich ; PhD^e ; Joachim W. Fluhr ; MD ; PhD^f ; Rainer Haag ; PhD^d ; Burkhard Kleuser ; PhD^c ; Ulrike Alexiev ; PhD^b ; Achim D. Gruber ; DVM ; PhD^a ; Lars Mundhenk ; DVM ; PhD^a ; ^{lars.mundhenk@fu-berlin.de}
• 关键词：CMS ; Fluorescence microscopy ; FLIM ; Mouse model ; Nile red
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：13
• 期：1
• 页码：317-327
• 全文大小：1923 K
• 卷排序：13

文摘

Inflammatory disorders of the skin pose particular therapeutic challenges due to complex structural and functional alterations of the skin barrier. Penetration of several anti-inflammatory drugs is particularly problematic in psoriasis, a common dermatitis condition with epidermal hyperplasia and hyperkeratosis. Here, we tested in vivo dermal penetration and biological effects of dendritic core-multishell-nanocarriers (CMS) in a murine skin model of psoriasis and compared it to healthy skin. In both groups, CMS exclusively localized to the stratum corneum of the epidermis with only very sporadic uptake by Langerhans cells. Furthermore, penetration into the viable epidermis of nile red as a model for lipophilic compounds was enhanced by CMS. CMS proved fully biocompatible in several in vitro assays and on normal and psoriatic mouse skin. The observations support the concept of CMS as promising candidates for drug delivery in inflammatory hyperkeratotic skin disorders in vivo.