Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides

详细信息    查看全文

• 作者：Daniela Verga ; Moritz Welter ; Andreas Marx ; ^{andreas.marx@uni-konstanz.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：DNA polymerase ; Oligonucleotide-modified nucleotide ; Rolling circle amplification ; Single-base resolution ; Naked-eye detection
• 刊名：Bioorganic & Medicinal Chemistry Letters
• 出版年：2016
• 出版时间：1 February 2016
• 年：2016
• 卷：26
• 期：3
• 页码：841-844
• 全文大小：700 K

文摘

DNA polymerases can efficiently and sequence selectively incorporate oligonucleotide (ODN)-modified nucleotides and the incorporated oligonucleotide strand can be employed as primer in rolling circle amplification (RCA). The effective amplification of the DNA primer by Φ29 DNA polymerase allows the sequence-selective hybridisation of the amplified strand with a G-quadruplex DNA sequence that has horse radish peroxidase-like activity. Based on these findings we develop a system that allows DNA detection with single-base resolution by naked eye.