Tumor suppressor RYBP harbors three nuclear localization signals and its cytoplasm-located mutant exerts more potent anti-cancer activities than corresponding wild type

详细信息    查看全文

• 作者：Kuan Tan¹ ; Xuan Zhang¹ ; Xiaojie Cong ; Bingren Huang ; Hong Chen ; ^{hchen_222@163.com} ; Deng Chen ; ^{chendeng2001@hotmail.com}
• 关键词：BCOR ; BCL6 corepressor ; DED ; death effector domain ; DEDD ; DED containing DNA-binding protein ; DISC ; death-inducing signaling complex ; EGFP ; enhanced green fluorescence protein ; FADD ; Fas-associated death domain ; FANK1 ; Fibronectin type III and ankyrin repeat domains 1 ; MDM2 ; Mouse double minute 2 ; NES ; nuclear export signal ; NLS ; nuclear localization signal ; NZF ; NPl4 Zinc Finger domain ; PCGF ; polycomb group ring fingers ; PRC1 ; polycomb repressive complex 1 ; RYBP ; Ring1 and YY1 Binding Protein
• 刊名：Cellular Signalling
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：29
• 期：Complete
• 页码：127-137
• 全文大小：1692 K
• 卷排序：29

文摘

RYBP contains three monopartite nuclear localization signals (NLSs) at its N-terminus. A cytoplasm-located mutant of RYBP (RYBPmut) was generated by replacing four basic amino acid residues in these NLSs. RYBPmut showed a more potent ability to bind to caspase 8, and to activate p53 than its wild type in human tumor cells. RYBPmut demonstrates more potent anti-tumor activities than its wild type, in both p53-dependent and independent manners.