The antigenic drift molecular basis of the H5N1 influenza viruses in a novel branch of clade 2.3.4

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• 作者：Lei Zhong^a ; ^b ; Qingqing Zhao^a ; ^b ; Kunkun Zhao^a ; ^b ; Xiaoquan Wang^a ; ^b ; Guo Zhao^a ; ^b ; Qunhui Li^a ; ^b ; Min Gu^a ; ^b ; Daxin Peng^a ; ^b ; Xiufan Liu^a ; ^b ; ^{xfliu@yzu.edu.cn" class="auth_mail} ; ^{xiufan2@126.com" class="auth_mail}
• 关键词：H5N1 ; Clade 2.3.4 ; Antigenic drift ; Antigenic site B ; Amino acid position 205
• 刊名：Veterinary Microbiology
• 出版年：25 June 2014
• 年：2014
• 卷：171
• 期：1-2
• 页码：23-30
• 全文大小：1105 K

文摘

H5N1 subtype influenza A virus has evolved into many HA clades since late 1990s. Six circulating H5N1 influenza viruses clustered to a novel branch in clade 2.3.4 and could escape vaccine protection, indicating their antigenic drift. Eleven amino acids substitutions in three antigenic sites of the hemagglutinin of these isolates were found when compared with the hemagglutinin of the primary viruses in clade 2.3.4. On the backbone of the novel isolates A/chicken/Northern China/k0602/2010, we generated a panel of recombinant viruses with HA mutations of restoring the primary vaccine strain Re-5's amino acid and homologous antisera to determine the role of these substitutions. The results of cross-HI assay, micro-neutralization assay and the antigen map of the mutated recombinant viruses showed that three substitutions in antigenic site B, especially D205K, are the major contributors to the antigenic drift of the novel branch of clade 2.3.4. Our study highlights the importance of surveillance of antigenic drift of H5N1 viruses for the control and preparedness of pandemic threats.