Basement membranes, microfibrils and β amyloid fibrillogenesis in Alzheimer's disease: high resolution ultrastructural findings

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• 作者：Inoue ; Sadayuki ; Kuroiwa ; Mie ; Kisilevsky ; Robert
• 关键词：Alzheimer's disease ; β ; amyloid fibril ; Basement membrane ; Microfibril ; Ultrastructure
• 刊名：Brain Research Reviews
• 出版年：1999
• 出版时间：April, 1999
• 年：1999
• 卷：29
• 期：2-3
• 页码：218-231
• 全文大小：5.97 M

文摘

It is known that β amyloid fibrils are deposited at the basement membrane of the cerebromicrovasculature in the brains of patients with Alzheimer's disease, and the assembly of the fibrils may be in continuation with the core of senile plaques. The fibrils accumulate in a manner similar to that in which microfibrils accumulate in the glomerular basement membrane of the rat kidney during long-term experimental diabetes, and in the alveolar-capillary basement membrane of the normal lung. β amyloid fibrils in-situ are known to be about 10 nm wide tubular structures and they closely resemble connective tissue microfibrils. Our recent high resolution ultrastructural studies combined with immunogold labeling demonstrated that β amyloid fibrils in-situ are indeed microfibril-like structures, and the β protein is associated with their surface in the form of loose assemblies of 1 nm wide flexible filaments. Thus, the result of this study indicates that in-situ a major component of the β amyloid deposit is the microfibril-like structure. The elucidation of the mechanism of cerebral β amyloid fibrillogenesis in Alzheimer's disease may therefore require understanding the mechanism of ‘normal' microfibrils biogenesis.